ESTCP Project #1011, Rhizosphere
Final Report
Appendix C. MPN Tubes
Media Preparation
MPP Buffer (same as described in Appendix B.)
Bushnell-Haas Negative Control (5 tubes/soil sample)
3.26 g Bushnell-Haas (BH) medium (Difco#0578-17-3)
1.0 L deionized H2O
(each tube contains 4.5 mL of BH medium)
Dextrose Positive Control (5 tubes/soil sample)
3.26 g BH medium
10.0 g Dextrose (Fisher #D-16)
1.0 L deionized H2O
(each tube contains 4.5 mL of Dextrose medium)
Motor Oil (40 tubes/soil sample)
3.26 g BH medium
1.0 L deionized H2O
(one drop Coastal 30W non-detergent motor oil added to each of 40 test tube
containing 4.5 mL BH medium/tube)
Reference: Walker, J.D., and R.R. Colwell.1976. Enumeration of petroleum-
degrading microorganisms. Appl. Environ. Microbiol. 31:198-207.
Vegetable Oil (40 tubes/soil sample)
3.26 g BH medium
1.0 L deionized H2O
(one drop Wesson vegetable oil added to each of 40 test tube containing 4.5 mL
BH medium/tube)
Sodium Benzoate (40 tubes/soil sample)
3.26 g BH medium
6.90 g Sodium Benzoate (Fisher#S-299)
1.0 L deionized H20
(each tube contains 4.5 mL of Sodium Benzoate medium)
Reference Modified from: Mesarch, M.B., and L. Nies. 1997. Modification of
heterotrophic plate counts for assessing the bioremediation potential of petroleum-
contaminated soils. Environ. Tech. 18:639-646.
Cyclohexanol (40 tubes/soil sample)
49
Previous Page
