ESTCP Project #1011, Rhizosphere
1. All glassware and dilution bottles should be autoclaved at 121C for 15 min prior to
cleaning or disposal. After autoclaving, glass pipets can be disposed of in waste glass
container. Used pipet tips and other materials may be disposed of in appropriate waste
containers after autoclaving. Rinse dilution bottles in sink, making sure to catch any soil
waste and glass beads in fine sieve. Rinse glass spreading bars, wash bars and dilution
bottles in dishwasher. Autoclave to reuse spreading bars. Refill dilution bottles with MPP
buffer and autoclave for later use.
1. Incubate plates in the dark at room temperature (25C).
Fungi: Martin's medium plates are to be read 3 and 10 days after inoculation.
Bacteria: 0.1 X TSA plates are to be read 2 and 7 days after inoculation.
Actinomycetes: Starch Casein plates are to be read 14 days after inoculation.
Values are calculated and reported as log10 CFU/g dry soil.