ESTCP Project #1011, Rhizosphere
Final Report
Actinomycetes: Starch Casein Medium
10.0 g soluble starch (Fisher #S-516)
0.30 g Casein Hydrolysate (Sigma #C-9386)
2.0 g KNO3
2.0 g NaCl
2.0 g K2HPO4
0.05 g MgSO4_7H2O
0.02 g CaCO3
0.01 g FeSO4_7H2O
15.0 g granulated agar
1.0 L distilled water
0.1 g cycloheximide in 1.0 mL methanol (added after autoclaving).
Boil agar, allow to cool slightly and adjust to pH 7 with HCl or NaOH.
Autoclave @ 121C for 15 min. After cooling to approximately 45C, add
cycloheximide.1
Reference: Wellington, E.M.H., and I.K. Toth, 1994. Actinomycetes. p. 269-290. In
R.W. Weaver (ed.). Methods of soil analysis. Part 2. Microbiological
and biochemical properties. SSSA Book Ser. 5. SSSA, Madison, WI.
Dilution bottles for serial dilution: MPP Buffer
0.65 g K2HPO4
0.35 g KH2PO4
0.10 g MgSO4_7H2O
1.0 mL Tween 80 (Baker #7-X257)2
1.0 L distilled water
Add 2 drops (0.1 mL) Tween 80 to -1 dilution bottles only.
2
Reference: Margesin, R., and F. Schinner. 1997. Laboratory bioremediation
experiments with soil from a diesel-oil contaminated site-significant role of
cold-adapted microorganisms and fertilizers. J. Chem. Tech. Biotechnol.
70:92-98.
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