Form Approved
REPORT DOCUMENTATION PAGE
OMB No. 0704-0188
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1. AGENCY USE ONLY (Leave blank)
2. REPORT DATE
3. REPORT TYPE AND DATES COVERED
December 1997
4. TITLE AND SUBTITLE
5. FUNDING NUMBERS
Evaluation of Commercial Enzyme Immunoassays for the
Field Screening of TNT and RDX in Water
6. AUTHORS
Philip G. Thorne and Karen F. Myers
7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES)
8. PERFORMING ORGANIZATION
REPORT NUMBER
U.S. Army Cold Regions Research and Engineering Laboratory
72 Lyme Road
Special Report 97-32
Hanover, New Hampshire 03755-1290
9. SPONSORING/MONITORING AGENCY NAME(S) AND ADDRESS(ES)
10. SPONSORING/MONITORING
AGENCY REPORT NUMBER
U.S. Army Environmental Center
Aberdeen Proving Ground, Maryland
SFIM-AEC-ET-CR-97057
For conversion of SI units to non-SI units of measurement consult Standard Practice for Use of the
11. SUPPLEMENTARY NOTES
International System of Units (SI), ASTM Standard E380-93, published by the American Society for Testing and Materials,
1916 Race St., Philadelphia, Pa. 19103.
12a. DISTRIBUTION/AVAILABILITY STATEMENT
12b. DISTRIBUTION CODE
Approved for public release; distribution is unlimited.
Available from NTIS, Springfield, Virginia 22161.
13. ABSTRACT (Maximum 200 words)
Water samples from 44 monitoring wells at three military installations were analyzed for the high explosives TNT
and RDX using immunoassay test kits. The accuracy and precision of the kit determinations were compared with
results obtained using the RP-HPLC, EPA Method 8330. Most of the kits achieved a 50% relative percent differ-
ence criterion over 85% of the time. One of the kits failed this test over half the time. Careful consideration must
be given to interferences that may be present and unique for each application.
14. SUBJECT TERMS
15. NUMBER OF PAGES
Chemical analysis
RDX
20
Environmental impact
Military research
16. PRICE CODE
TNT
17. SECURITY CLASSIFICATION
18. SECURITY CLASSIFICATION
19. SECURITY CLASSIFICATION
20. LIMITATION OF ABSTRACT
OF REPORT
OF THIS PAGE
OF ABSTRACT
UNCLASSIFIED
UNCLASSIFIED
UNCLASSIFIED
UL
Standard Form 298 (Rev. 2-89)
Prescribed by ANSI Std. Z39-18
298-102