APPENDIX F: EXTRACTION AND ANALYSIS: EPA METHOD
Method 8330 and Method 8095 can be downloaded in their entirety from the
EPA Internet site www.epa.gov.
The following sections were copied from EPA Method 8330 (EPA Method
1994), to support decisions when using this guide. The only change to the text is
the inclusion of Note 2.
A. SOIL EXTRACTION
Soil and Sediment Samples
1. Sample homogenization: Dry soil samples in air at room temperature or
colder to a constant weight, being careful not to expose the samples to direct
sunlight. Grind and homogenize the dried sample thoroughly in an acetonitrile-
rinsed mortar to pass a 30-mesh sieve.
2. NOTE 1: Soil samples should be screened by Method 8515 prior to grinding
in a mortar and pestle (See Safety Sec. 11.2)
3. NOTE 2: Some soil samples may require that the material is ground to a finer
particle size (30 mesh) than what is presented in this method to obtain an accept-
able level of homogenization (see Appendix B).
4. Sample extraction
5. Place a 2.0-g subsample of each soil sample in a 15-mL glass vial. Add 10.0
mL of acetonitrile, cap with Teflon-lined cap, vortex swirl for one minute, and
place in a cooled ultrasonic bath for 18 hours.
6. After sonication, allow sample to settle for 30 minutes. Remove 5.0 mL of
supernatant and combine with 5.0 mL of calcium chloride solution (Sec. 5.1.3) in
a 20-mL vial. Shake, and let stand for 15 minutes.
7. Place supernatant in a disposable syringe and filter through a 0.45-m Teflon
filter. Discard first 3 mL and retain remainder in a Teflon-capped vial for RP-
HPLC analysis as in Sec. 7.4.