Table 1. Performance Objectives
Type of
Primary
Expected
Actual Performance Objective Met?
Performance
Performance
Performance (Metric)
Objective
Criteria
Vegetation
Visual inspection of
Yes
Qualitative
established on plots
plots following seeding
and fertilizing
Relate success of
Use statistically valid
Yes. Using biomarker and growing degree-day
Quantitative
bioremediation to
time-series samples to
normalized data, statistical significance was
contaminant
develop equations to
shown for planted plots relative to un-planted
describe degradation
plots.
kinetics
Relate microbial
Measure degrader
Yes at Annette Island site. Significant effects
Quantitative
changes to
numbers via MPN
at one of three sites. Microbial data support
degradation
methods
chemical data results.
processes
Evaluate microbial
Use selective media
Yes at Annette Island site. Significant
Quantitative
population levels
techniques to compare
changes in fungal and microbial populations
and composition
fungal and bacterial
were related to plant and fertilizer treatments,
populations
respectively.
Reduce
Rate of degradation
Contaminant depletion rates, biomarker and
Quantitative
contaminant
growing-degree day normalized show greater
concentration
depletion of specific petroleum fractions
relative to unplanted plots.
Remediate site
Endpoint
Partial. Data show significantly greater rates for
Quantitative
concentrations not
planted treatments relative to un-planted
expected to become
treatments. Rhizosphere-enhance treatment is a
asymptotic
long-term treatment strategy useful to remote
sites, large areas, and locations/situations where
other alternatives do not exist.
3.2 Selection of Test Sites
To include a climatic gradation evaluation of rhizosphere-enhanced bioremediation, we chose
three sites on a south to north gradient of climatic conditions. Sites were selected to maximize
the potential for successful demonstrations and to meet DoD requirements associated with
ESTCP. We based our selection on the following criteria:
1. For maximizing future application and to gain the most information from the
demonstrations, we sought three sites, each in a different climatic zone in Alaska.
2. To appropriately address the DoD requirement and the objectives of ESTCP, each site
had a Native American association and was contaminated by DoD activities.
3. The SERDP- and Army EQT-funded research leading to this demonstration had been
conducted in well-drained (not saturated) soils. Accordingly, the sites chosen are not
wetlands and the demonstrations were on well-drained areas.
4. Each site needed to have an agreeable owner or Primary Responsible Party.
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